The quality of a silica gel is extremely important when you are using it for chromatography purposes, particularly when dealing with difficult separations of valuable compounds. You need to be confident about your recoveries. In chromatography, there are at least three physical properties that will influence your separation and that you need to consider when choosing your silica gel:

  • Particle shape (irregular or spherical )
  • Particle size distribution (tight or large)
  • Pore diameter (surface area)

These characteristics will directly influence crucial parameters involved in a successful chromatography:

  • Resolution (efficiency of separation & final purity)
  • Retention (which allows separation)
  • Capacity (maximal sample quantity and final recovery/yield)
  • Back-pressure (speed and pumps related issues)

 

Selection of Particle Size and Pore Diameters

The selection of the particle size distribution and pore diameter for your application varies on the type of chromatography being done. In addition, not all the 40-63um gels are the same, i.e the absence of so-called fines (particles below 40um) has a great impact on performance. 

 

How to Choose Between Irregular or Spherical Gels? 

Irregular silica gels are traditional in flash or gravity chromatography and have always been a spontaneous choice for preparative chromatography. Nowadays, spherical particles are now used increasingly. Cost is very important in preparative and process chromatography, and the use of monodisperse spherical particles with narrow particle size distribution is more expensive. It is possible in this case to use irregular silica but the separation may not provide the desired results. For these situations, There’s a more affordable class of spherical particles for preparative chromatography: SiliaSphere PC.

The advantage of using spherical materials over standard irregular material includes the following:

  • Increased efficiency of the eluent's flow characteristics
  • Better resolution
  • Ease of packing
  • Very high specific surface area
  • High mechanical stability under higher pressures

Various pore sizes are available in a wide range of particle sizes (20 to 200um). 

 

Irregular Gels    
product number      particle size       pore diameter 
  um mesh A
R10037L 75-150 100-200 30
R10130A 40-63 230-400 40
R10150A 60-120 325-625  40
R10140A 60-200 70-250 40
R10160A 120-200 70-125 40
R10180A 500-1000 18-35 40
R10110B 0-20 - 60
R10019B 10-30 - 60
R10017B 15-40 400-800 60
R10023B 20-45 - 60
R10030B (F60) 40-63 230-400 60
R12030B (P60) 40-63 230-400 60
R10530B (acid washed) 40-63 230-400 60
R10150B 60-120 325-625 60
R10040B (G60) 60-200 70-230 60
R10140B (GE60) 60-200 70-230 60
R10137B 75-150  100-200 60
R10057B 105-175 86-140 60
R10160B 120-200 70-125 60
R10170B 200-500 35-70 60
R10180B 500-1000 18-35 60
R10130D 40-63 230-400 90
R10140D 60-200 70-250 90
R10157D 105-175 86-140 90
R10170D 200-500 35-70 90
R10180D 500-1000 18-35 90
R10181D 800-1200 16-22 90
R10130H 40-63 230-400 150
R10150H 60-120 325-625 150
R10140H 60-200 70-250 150
R10157H 105-175 86-140 150
R10160H 120-200 70-125 150
R10170H 200-500 35-70 150
R10172H 250-500 35-60 150
R10180H 500-1000 18-35 150
R10181H 800-1200 16-22 150
R10130M 40-63 230-400 300
R10140M 60-200 70-250 300
R10170M 200-500 35-70 300
           
      
Spherical Gels    
product number        particle size        pore diameter 
  um mesh A
S10030B-A 50 300 60
S10072B-A 60 250 60
S10034B-A 75 200 60
S10040B-A 100 150 60
S10063B-A 150 100 60
S10066B-A 200 70 60
S10068B-A 300 50 60
S10020C 20-45 - 70
S10040C 75-200 70-200 70
S10030C 200-375 45-70 70
S10070C 200-500 35-70 70
S10095D-A 25 - 90
S10095E-A 20 - 100
S10020E 20-45 - 100
S10030E 40-75 200-400 100
S10040E 75-200 70-200 100
S10065E 150-250 60-100 100
S10070E 200-500 35-70 100
S10009G-A 20 - 120
S10030G-A 50 300 120
S10034G-A 75 200 120
S10040G-A 100 150 120
S10063G-A 150 100 120
S10020M 20-45 - 300
S10030M 40-75 200-400 300
S10040M 75-200 70-200 300
S10064M 150-200 70-200 300
S10070M 200-500 35-70 300
S10020P 20-45 - 500
S10030P 40-75 200-400 500
S10040P 75-200 70-200 500
S10070P 200-500 35-70 500
S10020S 20-45 - 800
S10030S 40-75 200-400 800
S10040S 75-200 70-200 800
S10070S 200-500 35-70 800
S10020T 20-45 - 1000
S10030T 40-75 200-400 1000
S10040T 75-200 70-200 1000
S10070T 200-500 35-70 1000